In the biopharmaceutical industry it is common to see protein hydrolysates in cell culture processes as part of a complete medium formulation or a fed-batch bioreactor process. It is well documented that hydrolysates can have a substantial positive impact on cell growth and/or protein production. However, given the undefined nature and the lot-to-lot variability associated with hydrolysates there exists a need to mitigate these risks with a chemically defined (CD) alternative that can maintain the desired performance. SAFC Biosciences® is now offering a new product that meets this goal and can quickly convert an undefined process to fully defined. The approach to solving this complex puzzle is discussed within, including details on how “active” hydrolysate fractions were found and the subsequent identification of the compounds contained within. The knowledge gained from this work was used to design EX-CELL® CD Hydrolysate Fusion, which is capable of replacing the functions of hydrolysates in many Chinese Hamster Ovary (CHO) cell systems.
What was covered in the webinar:
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Understanding of hydrolysate complexity and source of variability
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Analytical technologies used to characterized undefined components
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Impact of hydrolysate addition on CHO cell gene regulation and overall performance
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Product scale-up considerations
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Chemically defined application proofs
This webinar took place on November 18,2009
To download and view the webinar click on this link:
https://www1.gotomeeting.com/register/147249640
Mention priority code BWS9234SW2
